Microbiology
Non-invasive and real-time study of VOCs present in the headspace of cell cultures and breath printing with SUPER SESI-HRMS.
Identification of the organism present in the cell culture
Monitoring different stages of cell culture growth
Target specific metabolic pathways with isotopic labels
Study metabolic responses to drugs, stressful stimuli, or other specific conditions
Detection and identification of bacterial lung infections using breath analysis
Cell cultures VOCs monitoring with SUPER SESI-HRMS technology, 4 reasons:
1- Bioreactor Head Space analysis. SUPER SESI-HRMS can track growth-related metabolites in real-time, providing time-resolved abundance metabolites of 2 sec.
SUPER SESI-HRMS allows for the real-time monitoring and comprehensive characterization of microorganisms every 2 seconds.
SUPER SESI-HRMS can identify over 200 metabolites during culture growth in both shake flasks and bioreactor cultivations, each displaying a distinctive intensity profile. This capability plays a crucial role in advancing our comprehension of microorganism dynamics.
“The technique gently tracks biological processes at the metabolic level in vivo with a time resolution of less than one minute.”
Comprehensive Real-Time Analysis of the Yeast Volatilome; Comprehensive Real-Time Analysis of the Yeast Volatilome; Pablo M-L Sinues et al.
“SESI‑Orbitrap MS is a system for tracking microbial physiology without the need for sampling and for time‑resolved discoveries during metabolic transitions.”
Using off‑gas for insights through online monitoring of ethanol and baker’s yeast volatilome using SESI‑Orbitrap MS; Sci Rep 12, 12462 (2022); Lars M.Blank et al.
2- Cell culture head space volatiles analysis.
SUPER SESI-HRMS can detect and identify compounds from bacterial cultures, and the combination of these VOCs creates a unique pattern for each bacterial genus
SUPER SESI-HRMS also enables the monitoring of the kinetic profiles of hundreds of metabolic species emitted by the culture as they grow on agar plates.
” SESI-HRMS features a high gas-phase species’ sensitivity and a great selectivity driven by the high-resolution of the mass analyzer. This was accomplished in real time, without any sample preparation. These characteristics allowed for the first time to monitor the kinetic profiles of hundreds of metabolic species emitted by S. aureus and S. pneumoniae as they grew on agar plates. These hundreds of features rendered highly specific signatures, which enabled distinguishing the samples even at the strain level. “
Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites; iScience, Pablo Sinues et al.
3- SUPER SESI-HRMS can detect a wide range of analytes, including species of low vapor pressure at concentrations in the low parts-per-trillion range
SUPER SESI-HRMS offers a limit of detecction below 1 pptv. VOCs reflect the metabolic state of a cell and, thus, can be used to understand many biological processes
SUPER SESI-HRMS can identify individual species or serovars in a mixed culture, and the ratios of detected volatiles reflect the proportion of each bacterium in the mixture. This is because volatile organic compounds (VOCs) produced by bacteria generate characteristic odors that can be used for species or serovar identification
4- Application versatility, Customizable and easy implementation:
The equipment required for SUPER SESI-HRMS is readily available and can be easily interfaced with commercial high-resolution mass spectrometers. The workflow is relatively easy to implement and allows for customization based on specific research requirements.
The data generated from SUPER SESI-HRMS is compatible with standard software for direct-injection mass spectrometry-based metabolomics, making it easy to analyze and interpret the results.
SUPER SESI-HRMS has diverse applications in animal research, including studying disease progression, evaluating treatment efficacy, and monitoring environmental exposures. It can also aid in the development of new diagnostic tools and therapies.
CUSTOMER SUCCESS
Dr. Prof. Lars M. Blank - Institute of Applied Microbiology at RWTH Aachen University
“We showcases the SESI-Orbitrap MS system for tracking microbial physiology without the need for sampling and for time-resolved discoveries during metabolic transitions.”
Dr. Prof. Jangjiang Zhu - The Ohio State University, Ohio, USA.
“Our results suggested that the optimized SESI-HRMS method can be suitable forthe analysis of VFAs from gut microbes in a rapid, sensitive, and non-invasive manner.”
Dr. Prof. Jane Emily Hill - Research Chair in Breath Science and Technology, Canada
“Our data confirm the utility of SESI-MS in real-time identification of bacterial species or serovars in vitro, which, in the future, may play a promising clinical role in diagnosing infections.”
Dr. Prof. Pablo Sinues - University Children’s Hospital Basel, University of Basel, Switzerland
“ (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections […] Volatile organic compounds (VOCs) produced by growing S. aureus or S. pneumoniae cultures on blood agar plates were detected within minutes and allowed for the distinction of these two bacteria on a species and even strain level within hours.”
Dr. Prof. Xue Li - Jinan University, Guangzhou, China.
“[…] (SESI-HRMS) is an emerging analytical platform that enables accurate identification of complex mixtures of volatile organic cultures compounds in the headspace of cell cultures”
