Real-Time Chemical Characterization of Aerosols by Secondary Electrospray Ionization Coupled with High-Resolution Mass Spectrometry
T. Zivkovic Semren*, S. Majeed, M. Fatarova, C. Laszlo, C. Pak, S. Steiner, G. Vidal, A. Kuczaj, A. Mazurov, M. C. Peitsch, N. V. Ivanov, J. Hoeng, P. A. Guy
Inhalation as a route for administering drugs and dietary supplements has garnered significant attention over the last decade. We performed real-time analysis of aerosols using secondary electrospray ionization (SESI) technology interfaced with high-resolution mass spectrometry (HRMS)
Validating Discriminative Signatures for Obstructive Sleep Apnea in Exhaled Breath
B. Streckenbach, M. Osswald, S. Malesevic, R. Zenobi, and M. Kohler
Chemical analysis of exhaled breath have suggested the existence of an OSA-specific metabolic signature. Here, we validated this diagnostic approach and the proposed marker compounds, as well as their potential to reliably diagnose OSA.
Personalised therapeutic management of epileptic patients guided by pathway-driven breath metabolomics
Kapil Dev Singh, Martin Osswald, Victoria C. Ziesenitz, Mo Awchi, Jakob Usemann, Lukas L. Imbach, Malcolm Kohler, Diego García-Gómez, Johannes van den Anker, Urs Frey, Alexandre N. Datta & Pablo Sinues
Therapeutic management of epilepsy remains a challenge, since optimal systemic antiseizure medication (ASM) concentrations do not always correlate with improved clinical outcome and minimal side effects. We tested the feasibility of noninvasive real-time breath metabolomics as an extension of traditional therapeutic drug monitoring for patient stratification by simultaneously monitoring drug-related and drug-modulated metabolites.
Minimizing ion competition boosts volatile metabolome analysis by secondary electrospray ionization orbitrap mass spectrometry
Jiayi Lan, Jérôme Kaeslin, Giorgia Greter, Renato Zenobi
Here, we show that besides ion suppression, ion competition in the C-trap of Orbitrap instruments is another important factor that influences sensitivity and reproducibility of SESI-MS. Instead of acquiring the full mass-to-charge ratio (m/z) range, acquisition of consecutive m/z windows to minimize the ion competition effect allows the detection of more features.
Standardization procedures for real-time breath analysis by secondary electrospray ionization high-resolution mass spectrometry
K.Dev Singh, G. Tancev, F. Decrue, J. Usemann, R. Appenzeller, P.Barreiro, G. Jaumà, M. Macia Santiago, G. Vidal de Miguel, U.Frey, P. Sinues
Despite the attractiveness of breath analysis as a non-invasive means to retrieve relevant metabolic information, its introduction into routine clinical practice remains a challenge.