Mass spectrometric techniques for the analysis of volatile organic compounds emitted from bacteria

I. A. Ratiu, T. Ligor, Victor B.-Bintintan, B. Buszewski

Real-time mass spectrometric identification of metabolites characteristic of chronic obstructive pulmonary disease in exhaled breath.png

Abstract: Bacteria are the main cause of many human diseases. Typical bacterial identification methods, for example culture-based, serological and genetic methods, are time-consuming, delaying the potential for an early and accurate diagnosis and the appropriate subsequent treatment. Nevertheless, there is a stringent need for in situ tests that are rapid, noninvasive and sensitive, which will greatly facilitate timely treatment of the patients. This review article presents volatile organic metabolites emitted from various micro-organism strains responsible for common bacterial infections in humans. Additionally, the manuscript shows the application of different analytical techniques for fast bacterial identification. Details of these techniques are given, which focuses on their advantages and drawbacks in using for volatile organic components analysis.

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Mass-spectrometric detection of omega-oxidation products of aliphatic fatty acids in exhaled breath

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Secondary electrospray ionization proceeds via gas-phase chemical ionization