Dendritic cells (DCs) actively sample and present antigen to cells of the adaptive immune system and are thus vital for successful immune control and memory formation. Immune cell metabolism and function are tightly interlinked, and a better understanding of this interaction offers potential to develop immunomodulatory strategies. However, current approaches for assessing the immune cell metabolome are often limited by endpoint measurements, may involve laborious sample preparation, and may lack unbiased, temporal resolution of the metabolome. In this study, we present a novel setup coupled to a secondary electrospray ionization-high resolution mass spectrometric (SESIHRMS) platform allowing headspace analysis of immature and activated DCs in real-time with minimal sample preparation and intervention, with high technical reproducibility and potential for automation.

Improving the diagnosis and management of pediatric asthma is necessary. Breath analysis can help by assessing altered metabolism and disease-associated processes in a non-invasive manner. This study aimed to identify exhaled metabolic signatures using SUPER SESI - HRMS that can distinguish children with allergic asthma from healthy controls.

This application note highlights the potential of breath analysis in providing valuable insights into the exposure of lungs and the absorption of molecules directly into the bloodstream. The SUPER SESI - QExactive HF -EXHALION system was utilized for breath sample analysis, which recorded a volunteer's breath for 20 minutes after smoking a cigarette. The study captured insights into the kinetics of tobacco metabolites. This approach underscores the capability of breath analysis to provide real-time and valuable data on substance metabolism and exposure. It showcases a significant step forward in non-invasive diagnostic methodologies.

This study explores breath analysis as a tool for therapeutic drug monitoring (TDM) in clinical settings, focusing on valproic acid (VPA) in epilepsy treatment. It contrasts real-time and off-line mass spectrometry methods for analyzing exhaled compounds associated with VPA. Real-time analysis involves direct exhalation into a mass spectrometer, while off-line analysis uses a gas collection device.

Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that secondary electrospray ionization–high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections as a supportive system for current state-of-the-art diagnostics.

We are thrilled to announce a fantastic job opening at Diagnose Early (www.diagnoseearly.com), a pioneer in advanced technological research. Thanks to recent strategic investments, including the acquisition of several state-of-the-art SUPER SESI - Orbitrap breath analysis machines, DE is at the forefront of biomarker identification research.

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In this study, SUPER SESI-Orbitrap MS was used to analyze the time-resolved gas-phase volatile organic compounds (VOCs) of garlic, ramsons, and onion.

The aim of this study was to address the difficulties of characterizing heat-labile sulfur molecules from various Allium spp. In this, we will delve into the analytical method SUPER SESI-HRMS used in this study and its applications in the analysis of volatile compounds in garlic, onion, and ramsons.

SUPER SESI-HRMS was used to non-invasively and in real-time monitor the pharmacokinetics of ketamine, propofol, and valproic acid, along with their metabolites in mice. This approach allowed for obtaining real-time pharmacokinetic curves without the need to sacrifice the animals, representing a significant advance in terms of animal welfare and time and cost savings. 

Hendrik Mengers, Martin Zimmermann & Lars M. Blank from Aachen University monitored the volatilome of yeast during growth in a shake flasks bioreactor. Among many more metabolites, they caught the evolution of acetaldehyde and ethanol within seconds.

2021 has been a great year for us, despite the pandemic and all.
We're proud of servicing science a little more each year.

Thanks to SuperSESI we can finally see, accurately and in real time, what happens in our lungs after smoking.

This work from the Ohio State University shows the potential of the platform SUPER SESI (by Fossiliontech) - Orbitrap (by Thermo Fisher Scientific) on differentiating cancer types and monitoring their treatment.

Electrospray provides the best performance, but obtaining a stable signal can be tricky.
The new SUPER SESI-X, designed for the Thermo Orbitrap Exploris series, is fully automatic and is ready to incorporate intelligent control. This source will auto-pilot the electrospray to bring high performance and reliability.

We have developed this first pilot unit within the PERFECT LIFE project (funded by the European Union). It comprises a gas conditioning unit, a desorber, the Super SESI, the MS, and of course, the cartridges!

Converting data to knowledge can be a daunting task because breath samples are complex. This gap has been bridged with high-quality data and advanced metabolomics data analysis techniques.