Exhaled breath analysis in patients with potentially curative lung cancer undergoing surgery: a longitudinal study
Jonas Herth, Felix Schmidt, Sarah Basler, Noriane A Sievi and Malcolm Kohler
Exhaled breath analysis has emerged as a non-invasive and promising method for early detection of lung cancer, offering a novel approach for diagnosis through the identification of specific biomarkers present in a patient's breath…
Exhalomics as a noninvasive method for assessing rumen fermentationin dairy cows: Can exhaled-breath metabolomics replace rumen sampling?
M. Z. Islam, S. E. Räisänen, A. Schudel, K. Wang, T. He, C. Kunz, Y. Li, X. Ma, A. M. Serviento, Z. Zeng, F. Wahl, R. Zenobi, S. Giannoukos, and M. Niu.
Previously, we used secondary electrospray ionization-mass spectrometry (SESI-MS) to investigate the diurnal patterns and signal intensities of exhaled (EX) volatile fatty acids (VFA) of dairy cows. The current study aimed to validate the potential of an exhalomics approach for evaluating rumen fermentation. The experiment was conducted in a switchback design, with 3 periods of 9 d each, including 7 d for adaptation and 2 d for sampling. Four rumen-cannulated original Swiss Brown (Braunvieh) cows were randomly assigned to 1 of 2 diet sequences (ABA or BAB): (A) low starch (LS; 6.31% starch on a dry matter basis) and (B) high starch (HS; 16.2% starch on a dry matter basis). Feeding was once per day at 0830 h. Exhalome (with the GreenFeed System), and rumen samples were collected 8 times to represent every 3 h of a day, and EX-VFA and ruminal (RM)-VFA were analyzed using SESI-MS and HPLC, respectively. Furthermore, the VFA concentration in the gas phase (HR-VFA) was predicted based on RMVFA and Henry’s Law (HR) constants….
Metabolic trajectories of diabetic ketoacidosis onset described by breath analysis
Mo Awchi, Kapil Dev Singh, Sara Bachmann Brenner, Marie-Anne Burckhardt, Melanie Hess, Jiafa Zeng, Alexandre N Datta, Urs Frey, Urs Zumsteg, Gabor Szinnai, Pablo Sinues
Purpose: This feasibility study aimed to investigate the use of exhaled breath analysis to capture and quantify relative changes of metabolites during resolution of acute diabetic ketoacidosis under insulin and rehydration therapy…
Alternative electrolyte solutions for untargeted breath metabolomics using secondary-electrospray ionization high-resolution mass spectrometry
Cedric Wüthrich, Renato Zenobi, Stamatios Giannoukos
Rationale
Secondary-electrospray ionization (SESI) coupled with high-resolution mass spectrometry is a powerful tool for the discovery of biomarkers in exhaled breath. A primary electrospray consisting of aqueous formic acid (FA) is currently used to charge the volatile organic compounds in breath. To investigate whether alternate electrospray compositions could enable different metabolite coverage and sensitivities, the electrospray dopants NaI and AgNO3 were tested….
Systematic Study of Polymer Gas Sampling Bags for Offline Analysis of Exhaled Breath
Mateusz Fido, Simone Hersberger, Andreas Güntner, Renato Zenobi, Stamatios Giannoukos
Polymeric bags are a widely applied, simple, and cost-effective method for the storage and offline analysis of gaseous samples. Various materials have been used as sampling bags, all known to contain impurities and differing in their cost, durability, and storage capabilities. Herein, we present a comparative study of several well-known bag materials...
Data Collection of" Alternative Electrolyte Solutions for Untargeted Breath Metabolomics with Secondary-Electrospray Ionization High-Resolution Mass Spectrometry"
Cedric Wüthrich, Renato Zenobi, Stamatios Giannoukos
The mass spectrometer used in this study was an Orbitrap Q-Exactive Plus (Thermo Scientific) operated with the manufacturer’s standard control software (ExactiveTune, version 2.9, Thermo Scientific) and Xcalibur (version 4.1. 31.9, Thermo Scientific). Mass calibration was done according to the instrument manual and was always more recent than seven days according to specifications…
Advances in secondary electrospray ionization for breath analysis and volatilomics
Stamatios Giannoukos, Cedric Wüthrich
The measurement of volatile organic compounds (VOCs) originating from organisms allows continuous monitoring and a unique insight into the metabolism. One method offering the sensitivity to detect these VOCs is secondary electrospray ionization coupled to high-resolution mass spectrometry (SESI-HRMS). SESI was derived from electrospray ionization (ESI) and has found widespread application in clinical research and monitoring of animals. This review discusses the technical aspects behind SESI, the advancements, and the technical hurdles faced. Additionally, the recent advances in the applications of SESI in human and animal-centered research are presented.